{"id":8992,"date":"2025-05-01T20:04:38","date_gmt":"2025-05-01T17:04:38","guid":{"rendered":"https:\/\/hdlab.gr\/cosmetics-dermocosmetics\/%ce%b1%ce%bd%ce%b1%ce%bb%cf%85%cf%83%ce%b7-spf-in-vivo-in-vitro-%ce%b4%ce%bf%ce%ba%ce%b9%ce%bc%ce%b5%cf%83-%ce%b1%ce%bd%cf%84%ce%b7%ce%bb%ce%b9%ce%b1%ce%ba%ce%b7%cf%83-%cf%80%cf%81%ce%bf%cf%83\/"},"modified":"2026-03-23T10:37:01","modified_gmt":"2026-03-23T08:37:01","slug":"spf-testing-sunscreens","status":"publish","type":"page","link":"https:\/\/hdlab.gr\/en\/cosmetics-dermocosmetics\/spf-testing-sunscreens\/","title":{"rendered":"SPF Testing for Sunscreens &#8211; In Vivo &#038; In Vitro Methods (ISO Standards)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_section full_width=&#8221;stretch_row&#8221; css=&#8221;.vc_custom_1532167956213{margin-top: 0px !important;margin-bottom: 0px !important;padding-top: 20px !important;padding-bottom: 66px !important;}&#8221; el_class=&#8221;background-position-top-custom&#8221;][vc_row gap=&#8221;35&#8243;][vc_column width=&#8221;5\/12&#8243; 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Development (R&#038;D) \u2013 From Concept to Market<\/a><\/li>\n\t<li id=\"menu-item-9099\" class=\"menu-item menu-item-type-post_type menu-item-object-page rt-mega-menu-hover item-9099\"><a  href=\"https:\/\/hdlab.gr\/en\/cosmetics-dermocosmetics\/cosmetic-product-safety-assessment\/\">Cosmetic Product Safety Assessment (CPSR) \u2013 Regulation (EC) No 1223\/2009<\/a><\/li>\n\t<li id=\"menu-item-9100\" class=\"menu-item menu-item-type-post_type menu-item-object-page rt-mega-menu-hover item-9100\"><a  href=\"https:\/\/hdlab.gr\/en\/cosmetics-dermocosmetics\/microbiological-testing-cosmetics\/\">Microbiological Testing of Cosmetic Products (ISO 17516)<\/a><\/li>\n\t<li id=\"menu-item-9101\" class=\"menu-item menu-item-type-post_type menu-item-object-page rt-mega-menu-hover item-9101\"><a  href=\"https:\/\/hdlab.gr\/en\/cosmetics-dermocosmetics\/preservative-efficacy-testing\/\">Preservative Efficacy Testing (Challenge Test \u2013 ISO 11930)<\/a><\/li>\n\t<li id=\"menu-item-9102\" class=\"menu-item menu-item-type-post_type menu-item-object-page rt-mega-menu-hover item-9102\"><a  href=\"https:\/\/hdlab.gr\/en\/cosmetics-dermocosmetics\/cosmetic-stability-testing\/\">Cosmetic Stability Testing (ISO\/TR 18811)<\/a><\/li>\n\t<li id=\"menu-item-9103\" class=\"menu-item menu-item-type-post_type menu-item-object-page rt-mega-menu-hover item-9103\"><a  href=\"https:\/\/hdlab.gr\/en\/cosmetics-dermocosmetics\/patch-test-cosmetics\/\">Patch Test &#8211; 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From concept to compliant market launch.&#8221; font_container=&#8221;tag:p|font_size:15px|text_align:left|color:%23434343|line_height:26px&#8221; use_theme_fonts=&#8221;yes&#8221; css_animation=&#8221;none&#8221; css=&#8221;.vc_custom_1774255011020{margin-top: 0px !important;margin-bottom: 18px !important;padding-top: 0px !important;padding-bottom: 0px !important;}&#8221;]\r<!-- radiantthemes-custom-button -->\r<div class=\"radiantthemes-custom-button hover-style-one rt284006769  letter-spacing-0 font-weight-medium\"  data-button-direction=\"left\" data-button-fullwidth=\"false\" ><a style=\" color: #ffffff; line-height: 26px; font-size: 14px;\" class=\"radiantthemes-custom-button-main  vc_custom_1774255018646\" href=\"https:\/\/hdlab.gr\/en\/contact\/\"  title=\"\u0395\u03c0\u03b9\u03ba\u03bf\u03b9\u03bd\u03c9\u03bd\u03af\u03b1\" target=\"_self\"><div class=\"placeholder\">Contact us<\/div><\/a><\/div>\r<!-- radiantthemes-custom-button -->\r[\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column width=&#8221;7\/12&#8243; css=&#8221;.vc_custom_1532164210873{margin-top: 0px !important;margin-bottom: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221; offset=&#8221;vc_col-lg-8 vc_col-md-8 vc_col-xs-12&#8243;][vc_custom_heading text=&#8221;SPF Testing for Sunscreens &#8211; In Vivo &amp; In Vitro Methods (ISO Standards)&#8221; font_container=&#8221;tag:h2|font_size:30px|text_align:left|color:%232d2d2d|line_height:40px&#8221; use_theme_fonts=&#8221;yes&#8221; css_animation=&#8221;none&#8221; css=&#8221;.vc_custom_1773754145051{margin-top: 0px !important;margin-bottom: 0px !important;padding-top: 0px !important;padding-bottom: 0px !important;}&#8221;][vc_empty_space height=&#8221;15px&#8221;][vc_separator][vc_column_text css=&#8221;&#8221; el_class=&#8221;hdlabtext&#8221;]SPF evaluation is essential for <strong>consumer safety and claim substantiation<\/strong> under EU and international cosmetic regulations.<\/p>\n<p><strong>New ISO Standards for Sunscreen Testing (2024)<\/strong><\/p>\n<p>In line with continuous scientific advancement and alignment with the latest international standards, <strong>HD LAB<\/strong> applies the newly established ISO standards for sunscreen efficacy assessment:<\/p>\n<p><strong>ISO 23675:2024 \u2013 In Vitro Determination of SPF<\/strong><br \/>\nISO 23675:2024 defines a standardised methodology for the in vitro determination of SPF, providing reliable and reproducible laboratory-based evaluation of sunscreen performance, either as an alternative or complementary approach to in vivo testing.<\/p>\n<p><strong>ISO 23698:2024 \u2013 HDRS (Hybrid Diffuse Reflectance Spectroscopy)<\/strong><br \/>\nISO 23698:2024 introduces the HDRS method, combining in vivo and in vitro techniques for sunscreen assessment, enhancing result correlation, accuracy and contributing to the reduction of volunteer UV exposure.<\/p>\n<p>These methods are applied in accordance with European regulatory requirements and <strong>Cosmetics Europe guidelines<\/strong>, fully supporting <strong>PIF and CPSR documentation<\/strong>.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>In Vivo SPF Testing<\/strong><\/p>\n<p><em>(ISO 24444:2019)<\/em><\/p>\n<ul>\n<li>Volunteers: 10\u201320 subjects (Fitzpatrick phototypes I\u2013III)<\/li>\n<li>Application dose: <strong>2 mg\/cm\u00b2<\/strong><\/li>\n<li>UVB exposure: <strong>290\u2013320 nm<\/strong><\/li>\n<li>Measurement of MED (Minimal Erythema Dose)<\/li>\n<\/ul>\n<p><strong>Deliverables<\/strong><\/p>\n<ul>\n<li>ISO-certified SPF report<\/li>\n<li>Statistically validated SPF values<\/li>\n<li>Support for SPF 15, 30, 50+ and Very High Protection claims<\/li>\n<\/ul>\n<p><strong>In Vitro SPF &amp; UVA Testing<\/strong><\/p>\n<ul>\n<li><strong>ISO 24443:2021<\/strong> \u2013 UVA Protection Factor<\/li>\n<li><strong>ISO\/TR 20066:2020<\/strong> \u2013 In vitro SPF estimation<\/li>\n<li>PMMA substrates and UV spectrophotometry (290\u2013400 nm)<\/li>\n<\/ul>\n<p>Includes critical wavelength analysis (\u2265370 nm) and photostability assessment.[\/vc_column_text][\/vc_column][\/vc_row][\/vc_section]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_section full_width=&#8221;stretch_row&#8221; css=&#8221;.vc_custom_1532167956213{margin-top: 0px !important;margin-bottom: 0px !important;padding-top: 20px !important;padding-bottom: 66px !important;}&#8221; el_class=&#8221;background-position-top-custom&#8221;][vc_row gap=&#8221;35&#8243;][vc_column width=&#8221;5\/12&#8243; css=&#8221;.vc_custom_1531893806780{padding-left: 20px !important;}&#8221; offset=&#8221;vc_col-lg-4 vc_col-md-4 vc_col-xs-12&#8243;][vc_row_inner css=&#8221;.vc_custom_1532164808767{margin-top: 0px !important;margin-bottom: 0px !important;border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;border-left-color: #dfeaf2 !important;border-left-style: solid !important;border-right-color: #dfeaf2 !important;border-right-style: solid !important;border-top-color: #dfeaf2 !important;border-top-style: solid !important;border-bottom-color: #dfeaf2 !important;border-bottom-style: [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":8958,"menu_order":7,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-8992","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>SPF Testing for Sunscreens - In Vivo &amp; 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